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Some Amplification Needed
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Business New Haven
1/21/2002
By: BNH
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NEW HAVEN - Molecular Staging Inc. (MSI) has published a paper in the November issue of Nucleic Acids Research describing the application of the company's proprietary Rolling Circle Amplification Technology (RCAT) to detect nucleic acids on microarrays. The paper, entitled “Signal Amplification by Rolling Circle Amplification on DNA Microarrays,” outlines the benefits of RCAT as an on-chip signal amplification method. The distinctive properties of RCAT allow for multiplexing, or the ability to perform many assays simultaneously, without interference between the products of the amplification from each assay. This creates the opportunity for detecting both rare and abundant gene targets, while maintaining a high level of efficiency and specificity. The study observed that the amplification properties of RCAT enabled as few as 150 molecules bound to the surface of microarrays to be detected. This represents an 8,000-fold increase in detection sensitivity over hybridization, under the same conditions. This level of amplification indicates that RCAT operates at near maximal efficiency in a microarray format.
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